TY - JOUR ID - 38593 TI - Study the Interaction of Ni Complex of Tetradentate Schiff Base Ligand with HEN Egg White Lysozyme JO - Physical Chemistry Research JA - PCR LA - en SN - 2322-5521 AU - Sohrabi, Nasrin - AU - Rasouli, Nahid AU - Raissi, Marziyeh AD - Assistant Professor of phys. Chemistry,Academic member,Payame Noor University,Shahin shahr,Isfahan, Iran AD - Department of Chemistry, Payame Noor University (PNU), 19395-3697, Tehran, I.R.IRAN Y1 - 2017 PY - 2017 VL - 5 IS - 1 SP - 113 EP - 123 KW - Metal-Salen complex KW - Ni complex KW - Hen egg-white HEWL KW - Protein-binding DO - 10.22036/pcr.2017.38593 N2 - AbstractInteraction of Ni complex(Salen= N, N´-ethylene bis(salicylideneimine)) with hen egg-white lysozyme (HEWL) was studied by absorption spectroscopy, competitive binding study and thermal denaturation study. The protein binding affinity of Ni complex was found to be (3.0×103M−1). The binding plot obtained from the absorption titration data gives a binding constant of 2.4 (± 0.3)×103 M-1. It was found that the charge transfer band of the metal complex was perturbed in the presence of (HEWL). Thermal denaturation study of HEWL with Ni complex revealed the ΔTm of 5±0.2 °C. The thermodynamic parameters (ΔHº > 0 and ΔSº > 0) showed that the hydrophobic interaction leads to the increasing entropy which is brought about by interaction with the complex. The negative ΔGº values for interaction of HEWL with the Ni complex indicate the spontaneity of the complexation. UR - https://www.physchemres.org/article_38593.html L1 - https://www.physchemres.org/article_38593_6c6f77d5b523f657c04912dc33d9184b.pdf ER -