Nano Particle CORONA Formation; Rather than Proteins

Document Type: Regular Article


Shahid Beheshti University


Corona is a result of biological molecules and nanoparticles tend to each other and mainly refers to the case of proteins. The formation of the non-protein corona, i.e. carbohydrate and amino acids, and identifying the variations in their biological behavior were the purposes of this paper. The current study focused on silver nanoparticles (AgNPs), (20 and 120 nm), interaction with model small biomolecules, monosaccharides (glucose and fructose), and amino acids (histidine, cysteine, and tryptophan). The study considered the formation and composition of the corona as well as the characteristics and variations of carbohydrates/amino acids affected by AgNPs. Coronas were synthesized using the chemical reduction method, and their interactions with small biomolecules were monitored using response surface methodology (RSM). The results showed that treating glucose with smaller nanoparticles (20 nm) caused an increase in their size, agglomeration and aggregation, and a decrease in their homogeneity range. In the case of larger nanoparticles (120 nm), the glucose treatment caused the size to increase to ≥1 µm. Unlike glucose, fructose treatment had no influence on the size or stability of AgNPs.

Graphical Abstract

Nano Particle CORONA Formation; Rather than Proteins