TY - JOUR ID - 96007 TI - Nano Particle CORONA Formation; Rather than Proteins JO - Physical Chemistry Research JA - PCR LA - en SN - 2322-5521 AU - Mirzajani, Fateme AU - Pooya, Mahtab AD - Shahid Beheshti University Y1 - 2019 PY - 2019 VL - 7 IS - 4 SP - 849 EP - 857 KW - Amino acid KW - corona KW - DLS KW - Experimental Design KW - HPLC KW - Monosaccharide KW - Silver nanoparticle DO - 10.22036/pcr.2019.202628.1678 N2 - Corona is a result of biological molecules and nanoparticles tend to each other and mainly refers to the case of proteins. The formation of the non-protein corona, i.e. carbohydrate and amino acids, and identifying the variations in their biological behavior were the purposes of this paper. The current study focused on silver nanoparticles (AgNPs), (20 and 120 nm), interaction with model small biomolecules, monosaccharides (glucose and fructose), and amino acids (histidine, cysteine, and tryptophan). The study considered the formation and composition of the corona as well as the characteristics and variations of carbohydrates/amino acids affected by AgNPs. Coronas were synthesized using the chemical reduction method, and their interactions with small biomolecules were monitored using response surface methodology (RSM). The results showed that treating glucose with smaller nanoparticles (20 nm) caused an increase in their size, agglomeration and aggregation, and a decrease in their homogeneity range. In the case of larger nanoparticles (120 nm), the glucose treatment caused the size to increase to ≥1 µm. Unlike glucose, fructose treatment had no influence on the size or stability of AgNPs. UR - https://www.physchemres.org/article_96007.html L1 - https://www.physchemres.org/article_96007_7e2903d9510929466f80611f8ce25ba2.pdf ER -