The aggregation behavior of anionic Cobalt(II) 4,4′,4ʺ,4‴-tetrasulfonated phthalocyanine, [Co(TSPc)4-] was studied at its various concentrations and different ionic strengths using optical absorption and resonance light scattering (RLS) spectroscopies in 5 mM phosphate buffer, pH 7.0 at 25 °C. The results show no aggregation behavior at concentration range of 5.1 × 10-6-7.5 × 10-5 M. Also, the interaction of [Co(TSPc)4-] with calf thymus DNA (ct-DNA) was studied by UV-Vis absorption, fluorescence spectroscopies and thermal denaturation measurement. The binding constants were obtained at various ct-DNA concentrations using SQUAD software. The thermodynamic parameters were calculated by the van't Hoff equation. The results show that the process is entropy-driven. The fluorescence study represents the quenching effect of [Co(TSPc)4-] on bound ethidium bromide (EtBr) to ct-DNA.
Rasouli,N. and Sohrabi,N. (2016). A Study on the Aggregation and Calf Thymus DNA Binding Characteristics of Anionic Cobalt(II) Tetrasulfonated Phthalocyanine. Physical Chemistry Research, 4(1), 83-94. doi: 10.22036/pcr.2016.11976
MLA
Rasouli,N. , and Sohrabi,N. . "A Study on the Aggregation and Calf Thymus DNA Binding Characteristics of Anionic Cobalt(II) Tetrasulfonated Phthalocyanine", Physical Chemistry Research, 4, 1, 2016, 83-94. doi: 10.22036/pcr.2016.11976
HARVARD
Rasouli N., Sohrabi N. (2016). 'A Study on the Aggregation and Calf Thymus DNA Binding Characteristics of Anionic Cobalt(II) Tetrasulfonated Phthalocyanine', Physical Chemistry Research, 4(1), pp. 83-94. doi: 10.22036/pcr.2016.11976
CHICAGO
N. Rasouli and N. Sohrabi, "A Study on the Aggregation and Calf Thymus DNA Binding Characteristics of Anionic Cobalt(II) Tetrasulfonated Phthalocyanine," Physical Chemistry Research, 4 1 (2016): 83-94, doi: 10.22036/pcr.2016.11976
VANCOUVER
Rasouli N., Sohrabi N. A Study on the Aggregation and Calf Thymus DNA Binding Characteristics of Anionic Cobalt(II) Tetrasulfonated Phthalocyanine. PCR, 2016; 4(1): 83-94. doi: 10.22036/pcr.2016.11976
