In this paper, covalent immobilization of beta amylase enzyme on the surface of modified magnetic nano particles (ZnFe2O4@SiO2-NH2) is reported. For doing so, at first, the magnetic nanoparticles of ZnFe2O4 were synthesized by chemical co-precipitation method and then tetraethyl orthosilicate (TEOS) and 3-aminopropyltriethoxy silane (APTES) were used for modification of ZnFe2O4 nanoparticles with silica and amine groups (ZnFe2O4@SiO2-NH2). Then, the aminated surface of ZnFe2O4 nanoparticles was exposed to beta amylase immobilization using trichlorotriazine (TCT) as covalent agent. The immobilized beta-amylase enzyme was characterized by techniques such as Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), powder X-ray diffraction (XRD) and energy dispersive X-ray analysis (EDAX). The kinetics studies corroborate the Michaelis-Menten model and show much progress in the efficiency of immobilized enzyme compared to the free enzyme. Also, the thermal stability of the beta-amylase enzyme is increased after immobilization. By applying a magnetic active support, simple and facile separation of beta-amylase from the reaction mixture and higher catalytic activity is possible. The highest activity for immobilized beta-amylase enzyme is observed at pH and temperature of 7.0 and 40 °C, respectively.
Rasouli, N., Sohrabi, N., & Zamani, E. (2016). Influence of a Novel Magnetic Recoverable Support on Kinetic, Stability and Activity of Beta-amylase Enzyme. Physical Chemistry Research, 4(2), 271-283. doi: 10.22036/pcr.2016.13969
MLA
Nahid Rasouli; Nasrin Sohrabi; Elham Zamani. "Influence of a Novel Magnetic Recoverable Support on Kinetic, Stability and Activity of Beta-amylase Enzyme". Physical Chemistry Research, 4, 2, 2016, 271-283. doi: 10.22036/pcr.2016.13969
HARVARD
Rasouli, N., Sohrabi, N., Zamani, E. (2016). 'Influence of a Novel Magnetic Recoverable Support on Kinetic, Stability and Activity of Beta-amylase Enzyme', Physical Chemistry Research, 4(2), pp. 271-283. doi: 10.22036/pcr.2016.13969
VANCOUVER
Rasouli, N., Sohrabi, N., Zamani, E. Influence of a Novel Magnetic Recoverable Support on Kinetic, Stability and Activity of Beta-amylase Enzyme. Physical Chemistry Research, 2016; 4(2): 271-283. doi: 10.22036/pcr.2016.13969