Document Type : Regular Article
Department of Chemistry, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas 7915893144, Iran
Nasunin is the main phenol-based constituent of eggplant peels, be famed for its multiple advantages and antioxidant activity. α-lactalbumin (α-lac) is a milk protein with a great nutritional worthiness due to its necessary amino acids. Mechanism of interaction of α-lac with nasunin were investigated by in silico and spectroscopy studies. The results of the fluorescence quenching experiments demonstrated a reduction in the fluorescence parts by cause of bonding approach with fluorescing residues. The particle size of α-lac and nasunin complex is considerably more than the native protein. Far ultraviolet circular dichroism outcomes indicated that the second construction of the protein was rearranged in attending nasunin. Molecular docking pointed out that hydrogen binding has a significant influence on the constitution of nasunin-α-lac complex. The results suggested that α-lac could be a perfect carrier to nasunin loading for additional uses in nutrition field.