Document Type : Regular Article
Authors
1
PG and Research Department of Microbiology, Sri Akilandeswari Women’s College, Vandavasi-604408, Tamil Nadu, India. PG and Research Department of Microbiology, Indo-American College, Cheyyar-604407, Tamil Nadu, India
2
PG and Research Department of Microbiology, Sri Akilandeswari Women’s College, Vandavasi-604408, Tamil Nadu, India
3
PG and Research Department of Biochemistry, Indo-American College, Cheyyar-604407, Tamil Nadu, India
4
Department of Science and Humanities, St. Joseph College of Engineering, Sriperumbudur, Chennai-602117, Tamil Nadu, India
10.22036/pcr.2022.326373.2021
Abstract
In recent years, the green synthesis of nanoparticles using plant has attracted much attention due to its environment-friendly and economic aspects. The present study demonstrates an eco-friendly and low-cost method for antibacterial activity of biosynthesized silver nanoparticles [AgNPs] from phenolic compounds of plant extracts [Raphanus sativus and Curcuma longa]. GCMS and 1H NMR spectroscopy analyses showed the presence of phenolic compounds and alcohols groups of long-chain were also detected. Polyphenol compound like catechol and phenolic derivative like curcumin was capped with silver nanoparticles were proved by SEM analysis. However, the antibacterial potential of these green synthesized phytochemical capped AgNPs was exploited further in in-vivo studies. Albino mouse was induced with Multidrug-resistant E.coli and was treated with Ciprofloxacin as a conventional drug for standard groups and synthesized catechol-curcumin capped silver nanoparticles for test groups. Histopathology of albino mice kidney and bladder was performed after dosing the mice with specific concentrations of the nanoparticles for 3, 7, and 14 days. Photomicrographs of the kidney and bladder histology revealed cell regeneration, normal renal cortex, and glomerular tufts in the mice exposed to the nanoparticles.
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