Study the Interaction of Ni Complex of Tetradentate Schiff Base Ligand with HEN Egg White Lysozyme

Sohrabi, Nasrin -; Rasouli, Nahid; Raissi, Marziyeh

doi:10.22036/pcr.2017.38593

Study the Interaction of Ni Complex of Tetradentate Schiff Base Ligand with HEN Egg White Lysozyme

Document Type : Regular Article

Authors

Nasrin - Sohrabi ¹

Nahid Rasouli ²

Marziyeh Raissi ²

¹ Assistant Professor of phys. Chemistry,Academic member,Payame Noor University,Shahin shahr,Isfahan, Iran

² Department of Chemistry, Payame Noor University (PNU), 19395-3697, Tehran, I.R.IRAN

https://doi.org/10.22036/pcr.2017.38593

Abstract

Abstract
Interaction of Ni complex(Salen= N, N´-ethylene bis(salicylideneimine)) with hen egg-white lysozyme (HEWL) was studied by absorption spectroscopy, competitive binding study and thermal denaturation study. The protein binding affinity of Ni complex was found to be (3.0×103M−1). The binding plot obtained from the absorption titration data gives a binding constant of 2.4 (± 0.3)×103 M-1. It was found that the charge transfer band of the metal complex was perturbed in the presence of (HEWL). Thermal denaturation study of HEWL with Ni complex revealed the ΔTm of 5±0.2 °C. The thermodynamic parameters (ΔHº > 0 and ΔSº > 0) showed that the hydrophobic interaction leads to the increasing entropy which is brought about by interaction with the complex. The negative ΔGº values for interaction of HEWL with the Ni complex indicate the spontaneity of the complexation.

Graphical Abstract